Membrane Technology in the Production of Immunobiological Preparations Produced by the Branch Office of the FSBE «48 Central Scientific Research Institute» of the Ministry of Defence of Russia (Kirov)
https://doi.org/10.35825/2587-5728-2019-3-2-137-149
EDN: qoqlpz
Abstract
The researchers of the Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation (Kirov) organized theoretical and experimental studies on the introduction of a tangential filtration method for the separation of biological mixtures into the production of immunobiological preparations. The method of microfiltration in the tangential stream replaced the process of sedimentation at the stage of concoction of intermediate vaccines, reduced considerably the process time, and allowed to obtain suspensions from cultural liquid, substandard on an indicator of concentration of microbial cells. Along with this, the microfiltration method allowed to concentrate the cultures of Yersinia pestis of a vaccinal strain EV. In comparison with the centrifugal separation, the concentration of living microbial cells of a vaccinal strain of EV Y. pestis increased by one and a half times. The filtration in a tangential stream at the ASF-020 installation from the point of view of the production of the sporous product of anthrax vaccine STI-1 (in millions of doses), is 1.8 times more effective in comparison with the centrifugal separation. The membrane method allowed to reduce the duration of technological process. These membrane processes are used nowadays during the production of plague and anthrax vaccines, anthrax immunoglobulin, diagnostic medicines and during the sterilization of liquid nutrient mediums. This type of equipment for the sterilization of nutrient mediums can be considered as an alternative to the processes of the thermal sterilization of liquids and provides their biological and technological full functionality. Experiments on the use of the ceramic-metal filters sterilizing the air given for aeration, showed the decrease in duration of preparatory operations on 20 h and increase in the general operational opportunities of the system.
About the Authors
A. A. LeshchenkoRussian Federation
Andrey Anatolyevich Leshchenko. Leading Researcher of the Scientific and Research Department. Doctor of Technical Sciences, Professor
Oktyabrsky Avenue 119, Kirov 610000
D. A. Sharov
Russian Federation
Dmitry Alexandrovich Sharov. Chief of the Scientific and Research Department. Candidate of Technical Sciences
Oktyabrsky Avenue 119, Kirov 610000
V. V. Biryukov
Russian Federation
Vasily Vasilyevich Biryukov. Chief of the Scientific and Research Department - Deputy Head of the Unit. Candidate of Technical Sciences
Oktyabrsky Avenue 119, Kirov 610000
I. V. Kosenkov
Russian Federation
Ivan Viktorovich Kosenkov. Junior Researcher of the Scientific and Research Department
Oktyabrsky Avenue 119, Kirov 610000
I. P. Pogorelsky
Russian Federation
Ivan Petrovich Pogorelsky. Leading Researcher of the Scientific and Research Department. Doctor of Medical Sciences, Professor
Oktyabrsky Avenue 119, Kirov 610000
A. G. Lazykin
Russian Federation
Alexey Gennadievich Lazykin. Senior Researcher of the Scientific and Research Department. Candidate of Biological Sciences
Oktyabrsky Avenue 119, Kirov 610000
A. V. Ezhov
Russian Federation
Andrey Vladimirovich Ezhov. Senior Researcher of the Scientific and Research Department. Doctor of Medical Sciences, Senior Researcher
Oktyabrsky Avenue 119, Kirov 610000
S. V. Bagin
Russian Federation
Cergey Valerievich Bagin. Researcher of the Scientific and Research Department. Candidate of Technical Sciences
Oktyabrsky Avenue 119, Kirov 610000
S. V. Logvinov
Russian Federation
Sergey Vladimirovich Logvinov. Researcher of the Scientific and Research Department. Candidate of Biological Sciences
Oktyabrsky Avenue 119, Kirov 610000
D. A. Mokhov
Russian Federation
Dmitry Alexandrovich Mokhov. Researcher of the Scientific and Research Department. Candidate of Biological Sciences
Oktyabrsky Avenue 119, Kirov 610000
V. V. Krupin
Russian Federation
Vladimir Viktorovich Krupin. Deputy Head of the Department of the Scientific and Research Department. Candidate of Medical Sciences
Oktyabrsky Avenue 119, Kirov 610000
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Review
For citations:
Leshchenko A.A., Sharov D.A., Biryukov V.V., Kosenkov I.V., Pogorelsky I.P., Lazykin A.G., Ezhov A.V., Bagin S.V., Logvinov S.V., Mokhov D.A., Krupin V.V. Membrane Technology in the Production of Immunobiological Preparations Produced by the Branch Office of the FSBE «48 Central Scientific Research Institute» of the Ministry of Defence of Russia (Kirov). Journal of NBC Protection Corps. 2019;3(2):137-149. (In Russ.) https://doi.org/10.35825/2587-5728-2019-3-2-137-149. EDN: qoqlpz