Optimization of a Method for the Determination of a Mustard Gas Biomarker in Human Blood Plasma by Liquid Chromatography–Mass Spectrometry
https://doi.org/10.35825/2587-5728-2017-1-3-7-17
EDN: yoqccd
Abstract
A method for the determination of a mustard gas biomarker (an S-hydroxyethylthioethyl adduct with albumin) in blood plasma was optimized with the use of HPLC with tandem mass-spectrometric detection. This method is based on the hydrolysis of this adduct by the proteinase K enzyme with the formation of the following stable tripeptide with cysteine, proline, and phenylalanine: S-HETECys-Pro-Phe. The detection limit of mustard gas in human blood plasma was 2 ng/ml. The approach proposed was tested in the analysis of human blood plasma samples by the standard addition technique and also within the framework of the first official biomedical test carried out by the Organization for the Prohibition of Chemical Weapons (OPCW) in 2016, and it exhibited a good accuracy, reproducibility, and specificity of determination.
About the Authors
А. V. Braun
Federal State Budgetary Establishment «27 Scientific Centre» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Senior Researcher. Candidate of Chemical Sciences
Brigadirskii Lane 13, Moscow 105000
I. V. Rybalchenko
Federal State Budgetary Establishment «27 Scientific Centre» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Leading Researcher. Doctor of Chemical Sciences. Professor
Brigadirskii Lane 13, Moscow 105000
V. N. Fateenkov
Federal State Budgetary Establishment «27 Scientific Centre» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Chief of the Department. Candidate of Military Sciences
Brigadirskii Lane 13, Moscow 105000
М. А. Ponsov
Federal State Budgetary Establishment «27 Scientific Centre» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Senior Researcher. Candidate of Chemical Sciences
Brigadirskii Lane 13, Moscow 105000
Y. V. Stavitskay
Federal State Budgetary Establishment «27 Scientific Centre» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Researcher
Brigadirskii Lane 13, Moscow 105000
D. V. Oreshkin
Federal State Budgetary Establishment «27 Scientific Centre» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Researcher
Brigadirskii Lane 13, Moscow 105000
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Review
For citations:
Braun А.V., Rybalchenko I.V., Fateenkov V.N., Ponsov М.А., Stavitskay Y.V., Oreshkin D.V. Optimization of a Method for the Determination of a Mustard Gas Biomarker in Human Blood Plasma by Liquid Chromatography–Mass Spectrometry. Journal of NBC Protection Corps. 2017;1(3):7-17. (In Russ.) https://doi.org/10.35825/2587-5728-2017-1-3-7-17. EDN: yoqccd
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