The Development of Immunoenzyme Monoclonal Test Systems for Detection of Tularemia, Glanders, Melioidosis and Anthrax Agents
https://doi.org/10.35825/2587-5728-2017-1-2-21-27
EDN: yoqbzb
Abstract
The identification of causative agents of dangerous infectious diseases of bacterial origin is one of the main tasks of the biological protection of troops and the civilian population of the Russian Federation. This article is dedicated to the problem of the development of immunoenzyme monoclonal test system for detection of tularemia, glanders, melioidosis and anthrax agents. In order to achieve the above-mentioned goal, the hybridomas-producers of monoclonal antibodies to antigenic determinants of pathogens of tularemia, glanders, melioidosis and anthrax have been prepared. The pairs of monoclonal antibodies, the application of which in the composition of sensitin and immunoperoxidase conjugate provide maximum sensivity and specificity of enzyme immunoassay test, have been selected and the immunoenzyme monoclonal test systems for detection of tularemia, glanders, melioidosis and anthrax agents have been developed based on the above mentioned monoclonal antibodies. These developed test-systems can help to identify the pathogens of dangerous infectious diseases in microbial cultures, in probes taken from sick and dead people and animals and from environmental probes.
About the Authors
A. S. Tumanov
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Chief of the Branch. Candidate of Medical Sciences, Associate Professor
Oktyabrsky Avenue 119, Kirov 610000
K. A. Vorobyov
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Chief of the Department of Planning of Science and Research – Deputy Chief of the Branch. Doctor of Biological Sciences
Oktyabrsky Avenue 119, Kirov 610000
D. V. Pechenkin
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Pechenkin Denis Valeryevich. Chief of the Scientific and Research Department. Candidate of Medical Sciences
Oktyabrsky Avenue 119, Kirov 610000
G. D. Elagin
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Leading Researcher of the Scientific and Research Department. Candidate of Medical Sciences
Oktyabrsky Avenue 119, Kirov 610000
G. V. Kuklina
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Junior Researcher of the Scientific and Research Department. Candidate of Biological Sciences
Oktyabrsky Avenue 119, Kirov 610000
A. V. Eremkin
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Deputy Head of the Department – Chief of the Group of the Scientific and Research Department
Oktyabrsky Avenue 119, Kirov 610000
A. A. Kytmanov
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Researcher of the Scientific and Research Department. Candidate of Biological Sciences
Oktyabrsky Avenue 119, Kirov 610000
N. V. Bogacheva
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Chief of the Group of the Scientific and Research Department. Doctor of Medical Sciences, Associate Professor
Oktyabrsky Avenue 119, Kirov 610000
S. A. Shurupov
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Researcher of the Scientific and Research Department
Oktyabrsky Avenue 119, Kirov 610000
S. S. Ipatov
Branch Office of the Federal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of Defence of the Russian Federation
Russian Federation
Russian Federation
Junior Researcher of the Scientific and Research Department. Candidate of Biological Sciences
Oktyabrsky Avenue 119, Kirov 610000
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Review
For citations:
Tumanov A.S., Vorobyov K.A., Pechenkin D.V., Elagin G.D., Kuklina G.V., Eremkin A.V., Kytmanov A.A., Bogacheva N.V., Shurupov S.A., Ipatov S.S. The Development of Immunoenzyme Monoclonal Test Systems for Detection of Tularemia, Glanders, Melioidosis and Anthrax Agents. Journal of NBC Protection Corps. 2017;1(2):21-27. (In Russ.) https://doi.org/10.35825/2587-5728-2017-1-2-21-27. EDN: yoqbzb
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