nbsprotВестник войск РХБ защитыJournal of NBC Protection Corps2587-57283034-279127 Научный центр10.35825/2587-5728-2025-9-4-383-398nbsprot-434Research ArticleВООРУЖЕНИЯ И СРЕДСТВА ВОЙСК РХБ ЗАЩИТЫWEAPONS AND MEANS OF NBC PROTECTION TROOPНанопоровое секвенирование: теория и практикаNanopore sequensing: theory and practicehttps://orcid.org/0000-0002-9714-2085ПетровА. А.PetrovA. A.

Петров Александр Анатольевич. Начальник научно-исследовательского управления, д-р мед. наук.

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Aleksandr A. Petrov. Head of Research Department, Dr Sci. (Med.).

Oktyabrskaya Street, 11, Sergiev Posad-6 141306

48cnii@mil.ruhttps://orcid.org/0000-0001-7810-0675ЕрмаковА. М.ErmakovA. M.

Ермаков Артем Михайлович. Заведующий лабораторией исследований генома, канд. биол. наук.

142201, Московская обл., г. Серпухов, ул. Сиреневая, д. 8

142290, Московская обл., г. Пущино, ул. Институтская, д. 3

Artem M. Ermakov. Head of the Genome Research Laboratory, Cand. Sci. (Biol.).

Sirenevaya Street, 8, Serpukhov 142201, Moscow region

Institutskaya Street, 3, Pushchino 142290, Moscow Region

beoluchi@yandex.ruhttps://orcid.org/0009-0008-1858-8689БелозеровД. П.BelozerovD. P.

Белозеров Денис Петрович. Старший научный сотрудник.

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Denis P. Belozerov. Senior Researcher.

Oktyabrskaya Street, 11, Sergiev Posad-6 141306

48cnii@mil.ruhttps://orcid.org/0009-0003-0150-0998ПавлюковМ. Ю.PavlyukovM. Yu.

Павлюков Михаил Юрьевич. Старший научный сотрудник.

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Mikhail Yu. Pavlyukov. Senior Researcher.

Oktyabrskaya Street, 11, Sergiev Posad-6 141306

48cnii@mil.ruhttps://orcid.org/0009-0003-7760-6019СолдатенковаМ. И.SoldatenkovaM. I.

Солдатенкова Мария Игоревна. Младший научный сотрудник.

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Maria I. Soldatenkova. Junior researcher.

Oktyabrskaya Street, 11, Sergiev Posad-6 141306

48cnii@mil.ruКутаевД. А.KutaevD. A.

Кутаев Дмитрий Анатольевич. Заместитель начальника ФГБУ «48 ЦНИИ» Минобороны России по научной работе, канд. биол. наук.

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Dmitriy A. Kutaev. Deputy of Head of 48 Central Research Institute of the Ministry of Defence of the Russian Federation on scientific research, Cand. Sci. (Biol.).

Oktyabrskaya Street, 11, Sergiev Posad-6 141306

48cnii@mil.ruhttps://orcid.org/0000-0002-6742-3919БорисевичС. В.BorisevichS. V.

Борисевич Сергей Владимирович. Начальник ФГБУ «48 ЦНИИ» Минобороны России, д-р биол. наук, профессор, академик РАН.

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Sergey V. Borisevich. Head of 48 Central Research Institute of the Ministry of Defence of the Russian Federation, Academician of Russian Academy of Sciences, Dr Sci. (Biol.), Professor.

Oktyabrskaya Street, 11, Sergiev Posad-6 141306

48cnii@mil.ruФедеральное государственное бюджетное учреждение «48 Центральный научно-исследовательский институт» Министерства обороны Российской ФедерацииРоссия48 Central Research Institute of the Ministry of Defence of the Russian FederationRussian FederationООО «Нанопорус»; Федеральное государственное бюджетное учреждение науки «Институт теоретической и экспериментальной биофизики» Российской академии наукРоссияNanoporus LLC; Institute of Theoretical and Experimental Biophysics of the Russian Academy of SciencesRussian Federation20251601202694383398Copyright © Петров А.А., Ермаков А.М., Белозеров Д.П., Павлюков М.Ю., Солдатенкова М.И., Кутаев Д.А., Борисевич С.В., 20262026Петров А.А., Ермаков А.М., Белозеров Д.П., Павлюков М.Ю., Солдатенкова М.И., Кутаев Д.А., Борисевич С.В.Petrov A.A., Ermakov A.M., Belozerov D.P., Pavlyukov M.Y., Soldatenkova M.I., Kutaev D.A., Borisevich S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nbsprot.ru/jour/article/view/434

Основные моменты- проведено сравнительное исследование пригодности отечественной платформы «Нанопорус» и зарубежного аналога MinION для решения задач войск РХБ защиты;- доказана сопоставимая эффективность платформы «Нанопорус» после применения алгоритмов постобработки;- ключевые преимущества платформы «Нанопорус»: экономическая эффективность и возможность автономной работы.Актуальность. Обусловлена необходимостью развития отечественных технологий для оперативной идентификации патогенных биологических агентов (ПБА) в полевых условиях войсками РХБ защиты ВС РФ.Цель исследования – комплексный обзор теоретических основ и практического использования технологии нанопорового секвенирования, а также перспектив ее внедрения в войсках РХБ защиты ВС РФ.Материалы и методы. Сравнивали качество сборки геномов систем секвенирования «Нанопорус» (Нанопорус, Россия) и MinION (Oxford Nanopore Technologies, Великобритания). В качестве биологической модели использовали референсный штамм E. coli XL1-Blue (Евроген, Россия). Проведено экспериментальное нанопоровое секвенирование с выделением ДНК, подготовкой библиотек и последующей биоинформатической обработкой данных с использованием инструментов Flye и Medaka. Анализ полноты и точности сборки генома – по показателям N50 и Q-score.Результаты. Показано, что отечественная платформа «Нанопорус» обеспечивает высокое качество сборки геномов, сопоставимое с результатами, полученными на системе MinION, но при значительно меньших эксплуатационных затратах. Платформа компактна и пригодна для автономной работы в полевых условиях.Заключение. Платформа «Нанопорус» перспективна для интеграции в состав мобильных лабораторий войск РХБ защиты ВС РФ и может быть использована для решения задач идентификации ПБА.

HighlightsA comparative study was conducted on the suitability of the domestic "Nanoporus" platform and its foreign counterpart, MinION, for solving the tasks of the NBC Protection Corps.The comparable effectiveness of the "Nanoporus" platform was demonstrated after applying post-processing algorithms.Key advantages of the "Nanoporus" platform are its cost-effectiveness and capability for autonomous operation.Relevance. The study is determined by the need to develop domestic technologies for the rapid identification of pathogenic biological agents (PBA) in field conditions by the NBC Protection Corps.Purpose of the study is a comprehensive review of the theoretical foundations and practical applications of nanopore sequencing technology, as well as the prospects for its implementation in the NBC Protection Corps of the Russian Armed Forces.Materials and Methods. The quality of genome assemblies from the "Nanoporus" (Nanoporus, Russia) and MinION (Oxford Nanopore Technologies, UK) sequencing systems was compared. The reference strain E. coli XL1-Blue (Evrogen, Russia) was used as the biological model. Experimental nanopore sequencing was performed, including DNA extraction, library preparation, and subsequent bioinformatic data processing using the Flye and Medaka tools. Genome assembly completeness and accuracy were analyzed based on N50 and Q-score metrics.Results. It was shown that the domestic "Nanoporus" platform provides high genome assembly quality, comparable to the results obtained with the MinION system, but at significantly lower operational costs. The platform is compact and suitable for autonomous operation in field conditions.Conclusion. The "Nanoporus" platform is promising for integration into the mobile laboratories of the NBC Protection Corps of the Russian Armed Forces and can be used for solving PBA identification tasks.

MinIONOxford Nanopore Technologiesбиоинформатикагеномные исследованиядиагностикамолекулярная биологиянанопоровое секвенированиеподготовка образцовпрограммное обеспечениесистема секвенирования «Нанопорус»bioinformaticsdiagnosticsgenomic researchmolecular biologynanopore sequencingNanoporus sequencing systemsample preparationsoftwareMinIONOxford Nanopore TechnologiesФедеральное государственное бюджетное учреждение «48 Центральный научно-исследовательский институт» Министерства обороны Российской Федерации48 Central Scientific Research Institute of the Ministry of Defence of the Russian Federation.ReferencesWang Y, Zhao Y, Bollas A, Wang Y, Au KF. Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology. 2021;39(11):1348–65. https://doi.org/10.1038/s41587-021-01108-xWang Y, Zhao Y, Bollas A, Wang Y, Au KF. Nanopore sequencing technology, bioinformatics and applications. 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The authors declare that there are no conflicts of interest present.