nbsprotВестник войск РХБ защитыJournal of NBC Protection Corps2587-57283034-279127 Научный центр10.35825/2587-5728-2023-7-4-384-392nbsprot-336Research ArticleВооружение и средства войск РХБ защитыWeapons and Means of NBC Protection TroopsАпробация технологии конструирования средств экспресс-индикации новых особо опасных инфекцийApprobation of the Technology for Constructing Means of Express Indication of New Especially DangerousПетровА. А.PetrovA. A.

Петров Александр Анатольевич - Начальник научно-исследовательского управления, д-р мед. наук 

141306 ,г. Сергиев Посад-6, ул. Октябрьская, д. 11

Aleksandr A. Petrov -  Head of Research Department, Dr Sci. (Med.)

Oktyabrskaya Street 11, Sergiev Posad 141306

48cnii@mil.ruКазанцевА. В.KazantsevA. V.

Казанцев Алексей Васильевич - Старший научный сотрудник, канд. биол. наук

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Aleksey V. Kazantsev - Leading researcher, Cand. Sci. (Biol.)

Oktyabrskaya Street 11, Sergiev Posad 141306

48cnii@mil.ruПанферовК. А.PanferovK. A.

Панферов Кирилл Алексеевич - Старший научный сотрудник

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Kirill A. Panferov -  Leading researcher

Oktyabrskaya Street 11, Sergiev Posad 141306

48cnii@mil.ruЧисловА. А.ChislovA. A.

Числов Антон Александрович - Научный сотрудник.

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Anton A. Chislov - Researcher

Oktyabrskaya Street 11, Sergiev Posad 141306

48cnii@mil.ruКовальчукЕ. А.KovalchukE. A.

Ковальчук Елена Анатольевна - Научный сотрудник, канд. мед. наук

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Elena A. Kovalchuk - Researcher, Cand. Sci. (Biol.)

Oktyabrskaya Street 11, Sergiev Posad 141306

48cnii@mil.ruКутаевД. А.KutaevD. A.

Кутаев Дмитрий Анатольевич - Заместитель начальника ФГБУ «48 ЦНИИ» Минобороны России по научной работе, канд. биол. наук 

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Dmitriy A. Kutaev - Deputy of Head of Federal State Budgetary Establishment «48 Central Scientific Research Institute Research» of the Ministry of Defense of the Russian Federation on scientific research, Cand. Sci. (Biol).

Oktyabrskaya Street 11, Sergiev Posad 141306

48cnii@mil.ruhttps://orcid.org/0000-0002-6742-3919БорисевичС. В.BorisevichS. V.

Борисевич Сергей Владимирович - Начальник ФГБУ «48 ЦНИИ» Минобороны России, д-р биол. наук, профессор, академик РАН

141306, г. Сергиев Посад-6, ул. Октябрьская, д. 11

Sergey V. Borisevich -  Head of Federal State Budgetary Establishment «48 Central Scientific Research Institute Research» of the Ministry of Defense of the Russian Federation, Academician of Russian Academy of Sciences, Dr Sci. (Biol.), Professor

Oktyabrskaya Street 11, Sergiev Posad 141306

48cnii@mil.ruФедеральное государственное бюджетное учреждение «48 Центральный научно-исследовательский институт» Министерства обороныРоссияFederal State Budgetary Establishment «48 Central Scientific Research Institute» of the Ministry of DefenseRussian Federation20230304202474384392Copyright © Петров А.А., Казанцев А.В., Панферов К.А., Числов А.А., Ковальчук Е.А., Кутаев Д.А., Борисевич С.В., 20242024Петров А.А., Казанцев А.В., Панферов К.А., Числов А.А., Ковальчук Е.А., Кутаев Д.А., Борисевич С.В.Petrov A.A., Kazantsev A.V., Panferov K.A., Chislov A.A., Kovalchuk E.A., Kutaev D.A., Borisevich S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.nbsprot.ru/jour/article/view/336

Катастрофическая пандемия особо опасного коронавируса SARS-CoV-2 в 2020–2022 гг. и неожиданное распространение в 2022 г. из Африки возбудителя оспы обезьян, демонстрируют необходимость адекватного реагирования на биологические угрозы, имеющие в качестве источника происхождения экзотические инфекции, преодолевающие межвидовой барьер между животными и человеком и обладающие в отношении последнего высокими показателями вирулентности и контагиозности. Цель работы – создать технологию конструирования средств экспресс-индикации новых особо опасных и экзотических инфекций, позволяющую в сжатые сроки разработать генодиагностическое средство, оценить его характеристики и развернуть широкомасштабное производство. Материалы и методы. Использовали технологии конструирования средств экспресс-индикации новых особо опасных и экзотических инфекций на основе методов полимеразной цепной реакции в реальном времени (ОТ-ПЦР-РВ-Грипп/Коронавирус), пригодного для мультиплексной идентификации РНК коронавирусов. Обсуждение результатов. Разработанная технология конструирования средств экспресс-индикации новых особо опасных и экзотических инфекций успешно апробирована на лабораторной базе ФГБУ «48 ЦНИИ» МО РФ на примере конструирования «Набора реагентов для выявления РНК коронавирусов SARS-CoV, MERS-CoV, SARS-CoV-2 и вируса гриппа А методом полимеразной цепной реакции в реальном времени (ОТ-ПЦР-РВ-Грипп/Коронавирус)», пригодного для мультиплексной идентификации РНК коронавирусов. Вывод. В результате проведенных исследований по оценке имеющегося на лабораторной базе ФГБУ «48 ЦНИИ» МО РФ оборудования, адаптации и внедрения в практику ключевых производственных процессов, разработки и изготовления реагентных средств экспресс-индикации, а также апробации технологии конструирования средств экспресс-индикации новых особо опасных и экзотических инфекций на примере конструирования набора реагентов «ОТ-ПЦР-РВ-Грипп/Коронавирус» создана генодиагностическая платформа для разработки реагентных средств экспресс-индикации новых особо опасных и экзотических инфекций.

Catastrophic pandemic of the particularly dangerous coronavirus SARS-CoV-2 in 2020–2022 and the unexpected  spread of the monkeypox pathogen from Africa in 2022, demonstrate the need for an adequate response to biological  threats that have exotic infections as their source, overcome the interspecies barrier between animals and humans  and have high rates of virulence and contagiousness in relation to the latter. The purpose of the article is to create  a technology for constructing means for the express indication of new especially dangerous and exotic infections,  which makes it possible to quickly develop a gene diagnostic tool, evaluate its characteristics and launch large-scale  production. Materials and methods. The authors used technologies for constructing means for express indication  of new especially dangerous and exotic infections based on real-time polymerase chain reaction (RT-PCR-RT-Flu/ Coronavirus) methods, suitable for multiplex identification of coronavirus RNA. The discussion of the results. The  developed technology for constructing means for express indication of new especially dangerous and exotic infections  was successfully tested at the laboratory base of the FSBЕ «48 Central Research Institute» of the Ministry of Defense  of the Russian Federation using the example of designing a «Set of reagents for detecting the RNA of coronaviruses  SARS-CoV, MERS-CoV, SARS-CoV-2 and virus influenza A by real-time polymerase chain reaction (RT-PCR-RT-Flu/Coronavirus)», suitable for multiplex identification of coronavirus RNA. Conclusion. As a result of the research  carried out to evaluate the equipment available at the laboratory base of the FSBЕ «48 Central Research Institute» of  the Ministry of Defense of the Russian Federation, the adaptation and implementation of key production processes,  the development and production of express-indication reagents, as well as testing the technology for constructing  express-indication means for new especially dangerous and exotic infections, using the example of designing a set of  RT-PCR-RV-Flu/Coronavirus reagents, a gene diagnostic platform was created for the development of reagents for the  express indication of new especially dangerous and exotic infections.

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The authors declare that there are no conflicts of interest present.